Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y

Neurogenetics. 2013 Feb;14(1):63-70. doi: 10.1007/s10048-012-0350-9. Epub 2012 Dec 9.


The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and clinical significance. An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype. This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders. Here, we present a comprehensive assessment of the SH-SY5Y cytogenomic profile. Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain Neoplasms / genetics*
  • Brain Neoplasms / metabolism
  • Brain Neoplasms / pathology
  • Cell Line, Tumor
  • Comparative Genomic Hybridization
  • Cytogenetic Analysis
  • Gene Dosage
  • Genomics / methods
  • Humans
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Models, Theoretical
  • Neuroblastoma / genetics*
  • Neuroblastoma / metabolism
  • Neuroblastoma / pathology*
  • Neurons / cytology
  • Neurons / metabolism*
  • Neurons / pathology