The role of GW182 proteins in miRNA-mediated gene silencing

Adv Exp Med Biol. 2013;768:147-63. doi: 10.1007/978-1-4614-5107-5_9.

Abstract

GW182 family proteins are essential for microRNA-mediated gene silencing in animal cells. They are recruited to miRNA targets through direct interactions with Argonaute proteins and promote target silencing. They do so by repressing translation and enhancing mRNA turnover. Although the precise mechanism of action of GW182 proteins is not fully understood, these proteins have been shown to interact with the cytoplasmic poly(A)-binding protein (PABP) and with the PAN2-PAN3 and CCR4-NOT deadenylase complexes. These findings suggest that GW182 proteins function as scaffold proteins for the assembly of the multiprotein complex that silences miRNA targets.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Argonaute Proteins / genetics*
  • Argonaute Proteins / metabolism
  • Autoantigens / chemistry
  • Autoantigens / genetics*
  • Autoantigens / metabolism
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Nuclear Receptor Subfamily 4, Group A, Member 2 / genetics
  • Nuclear Receptor Subfamily 4, Group A, Member 2 / metabolism
  • Poly(A)-Binding Proteins / genetics
  • Poly(A)-Binding Proteins / metabolism
  • Protein Binding
  • Protein Biosynthesis
  • Protein Isoforms / chemistry
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Protein Structure, Tertiary
  • RNA Interference*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism
  • Ribonucleases / genetics
  • Ribonucleases / metabolism

Substances

  • Argonaute Proteins
  • Autoantigens
  • Drosophila Proteins
  • MicroRNAs
  • NR4A2 protein, human
  • Nuclear Receptor Subfamily 4, Group A, Member 2
  • Poly(A)-Binding Proteins
  • Protein Isoforms
  • RNA, Messenger
  • RNA-Binding Proteins
  • TNRC6A protein, human
  • CCR4 protein, Drosophila
  • Ribonucleases