The Penicillium echinulatum secretome on sugar cane bagasse

PLoS One. 2012;7(12):e50571. doi: 10.1371/journal.pone.0050571. Epub 2012 Dec 5.


Plant feedstocks are at the leading front of the biofuel industry based on the potential to promote economical, social and environmental development worldwide through sustainable scenarios related to energy production. Penicillium echinulatum is a promising strain for the bioethanol industry based on its capacity to produce large amounts of cellulases at low cost. The secretome profile of P. echinulatum after grown on integral sugarcane bagasse, microcrystalline cellulose and three types of pretreated sugarcane bagasse was evaluated using shotgun proteomics. The comprehensive chemical characterization of the biomass used as the source of fungal nutrition, as well as biochemical activity assays using a collection of natural polysaccharides, were also performed. Our study revealed that the enzymatic repertoire of P. echinulatum is geared mainly toward producing enzymes from the cellulose complex (endogluganases, cellobiohydrolases and β-glucosidases). Glycoside hydrolase (GH) family members, important to biomass-to-biofuels conversion strategies, were identified, including endoglucanases GH5, 7, 6, 12, 17 and 61, β-glycosidase GH3, xylanases GH10 and GH11, as well as debranching hemicellulases from GH43, GH62 and CE2 and pectinanes from GH28. Collectively, the approach conducted in this study gave new insights on the better comprehension of the composition and degradation capability of an industrial cellulolytic strain, from which a number of applied technologies, such as biofuel production, can be generated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomass
  • Chromatography, Liquid
  • Microscopy, Electron, Scanning
  • Penicillium / growth & development
  • Penicillium / isolation & purification*
  • Penicillium / metabolism
  • Saccharum / microbiology*
  • Tandem Mass Spectrometry

Grant support

This research was supported by grants from FAPESP (2008/58037-9) and CNPq (475022/2011-4 e 310177/2011-1). TMA received a fellowship from FAPESP (2010/11499-1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.