Photosystem II: the water-splitting enzyme of photosynthesis

Cold Spring Harb Symp Quant Biol. 2012:77:295-307. doi: 10.1101/sqb.2012.77.014472. Epub 2012 Dec 12.

Abstract

The oxygen in our atmosphere is derived and maintained by the water-splitting process of photosynthesis. The enzyme that facilitates this reaction and therefore underpins virtually all life on our planet is known as photosystem II (PSII), a multisubunit enzyme embedded in the lipid environment of the thylakoid membranes of plants, algae, and cyanobacteria. During the past 10 years, crystal structures of a 700-kDa cyanobacterial dimeric PSII complex have been reported with ever-increasing improvement in resolution--the latest at 1.9 Å. Thus, the organizational and structural details of its many subunits and cofactors are now well understood. The water-splitting site was revealed as a cluster of four Mn ions and one Ca ion surrounded by amino-acid side chains, of which seven provide ligands to the metals. The metal cluster is organized as a cubane-like structure composed of three Mn ions and the one Ca2+ ion linked by oxo bonds. The fourth Mn is attached to the cubane via one of its bridging oxygens together with another oxo bridge to an Mn ion of the cubane. The overall structure of the catalytic site provides a framework to propose a mechanistic scheme for the water-splitting process and gives a blueprint for the development of catalysts that mimick the reaction in an artificial chemical system as a means to generate solar fuels.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Models, Biological
  • Oxidation-Reduction
  • Photosynthesis
  • Photosystem II Protein Complex / chemistry
  • Photosystem II Protein Complex / metabolism*
  • Plants / enzymology*
  • Water / metabolism*

Substances

  • Photosystem II Protein Complex
  • Water