Ciliated cells of pseudostratified airway epithelium do not become mucous cells after ovalbumin challenge

Am J Respir Cell Mol Biol. 2013 Mar;48(3):364-73. doi: 10.1165/rcmb.2012-0146OC. Epub 2012 Dec 13.


Mucous cell metaplasia is a hallmark of airway diseases, such as asthma and chronic obstructive pulmonary disease. The majority of human airway epithelium is pseudostratified, but the cell of origin of mucous cells has not been definitively established in this type of airway epithelium. There is evidence that ciliated, club cell (Clara), and basal cells can all give rise to mucus-producing cells in different contexts. Because pseudostratified airway epithelium contains distinct progenitor cells from simple columnar airway epithelium, the lineage relationships of progenitor cells to mucous cells may be different in these two epithelial types. We therefore performed lineage tracing of the ciliated cells of the murine basal cell-containing airway epithelium in conjunction with the ovalbumin (OVA)-induced murine model of allergic lung disease. We genetically labeled ciliated cells with enhanced Yellow Fluorescent Protein (eYFP) before the allergen challenge, and followed the fate of these cells to determine whether they gave rise to newly formed mucous cells. Although ciliated cells increased in number after the OVA challenge, the newly formed mucous cells were not labeled with the eYFP lineage tag. Even small numbers of labeled mucous cells could not be detected, implying that ciliated cells make virtually no contribution to the new goblet cell pool. This demonstrates that, after OVA challenge, new mucous cells do not originate from ciliated cells in a pseudostratified basal cell-containing airway epithelium.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / immunology
  • Animals
  • Asthma / pathology
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Epithelial Cells / cytology*
  • Epithelial Cells / drug effects
  • Goblet Cells / cytology
  • Goblet Cells / drug effects
  • Hyperplasia / pathology
  • Male
  • Metaplasia / pathology
  • Mice
  • Mice, Inbred C57BL
  • Ovalbumin / pharmacology*
  • Respiratory Mucosa / cytology*
  • Respiratory Mucosa / drug effects
  • Stem Cells / cytology*
  • Stem Cells / drug effects


  • Allergens
  • Ovalbumin