Identification of novel interacting partners of Sirtuin6

PLoS One. 2012;7(12):e51555. doi: 10.1371/journal.pone.0051555. Epub 2012 Dec 11.

Abstract

SIRT6 is a member of the Sirtuin family of histone deacetylases that has been implicated in inflammatory, aging and metabolic pathways. Some of its actions have been suggested to be via physical interaction with NFκB and HIF1α and transcriptional regulation through its histone deacetylase activity. Our previous studies have investigated the histone deacetylase activity of SIRT6 and explored its ability to regulate the transcriptional responses to an inflammatory stimulus such as TNFα. In order to develop a greater understanding of SIRT6 function we have sought to identify SIRT6 interacting proteins by both yeast-2-hybrid and co-immunoprecipitation studies. We report a number of interacting partners which strengthen previous findings that SIRT6 functions in base excision repair (BER), and novel interactors which suggest a role in nucleosome and chromatin remodeling, the cell cycle and NFκB biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle / genetics
  • Chromatin Assembly and Disassembly / genetics
  • DNA Repair / genetics
  • HEK293 Cells
  • Humans
  • Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
  • NF-kappa B / metabolism
  • Nucleosomes* / genetics
  • Nucleosomes* / metabolism
  • Protein Binding*
  • Proteomics*
  • Sirtuins* / genetics
  • Sirtuins* / metabolism

Substances

  • HIF1A protein, human
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • NF-kappa B
  • Nucleosomes
  • SIRT6 protein, human
  • Sirtuins

Grant support

This work was fully funded by GlaxoSmithKline (GSK) and the authors were all employees of GSK at the time the work was done. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.