Cyclooxygenase-1 mediated platelet reactivity in young male smokers

Clin Appl Thromb Hemost. 2014 May;20(4):371-7. doi: 10.1177/1076029612466284. Epub 2012 Dec 14.


Background: The purpose of this study is to investigate the acute and chronic effects of cigarette smoking on cyclooxygenase- 1(COX-1)-mediated platelet reactivity among cigarette smokers.

Methods: The levels of collagen-induced platelet aggregation, platelet COX-1 activity, and expressions were compared between smokers and age-matched nonsmokers. In smokers, the acute effects of cigarette smoking were assessed by repeating these measurements an hour after smoking.

Results: Twenty-five smokers and age-matched nonsmokers (all men; mean age, 29 years) were studied. Collagen-induced platelet aggregation and plasma/urinary thromboxane B2 (TXB2) and 11-dehydroxythromboxane B2 levels were higher in cigarette smokers compared to nonsmokers. Greater expression of platelet COX-1 was observed in smokers than in nonsmokers. Among smokers, collagen-induced platelet aggregation correlated positively with platelet volume and circulating nicotine and cotinine concentrations. The levels of plasma/urinary TXB2 were significantly increased an hour after cigarette smoking.

Conclusion: Cigarette smoking aggravates COX-1-mediated platelet reactivity in young, otherwise healthy, smoking men.

Keywords: atherosclerosis; platelet dysfunction; platelet function; vascular disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biomarkers / blood
  • Biomarkers / urine
  • Blood Platelets / metabolism*
  • Cyclooxygenase 1 / blood*
  • Humans
  • Male
  • Platelet Aggregation
  • Smoking / blood*
  • Smoking / urine
  • Thromboxane B2 / blood
  • Thromboxane B2 / urine


  • Biomarkers
  • Thromboxane B2
  • Cyclooxygenase 1
  • PTGS1 protein, human