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. 2013 Feb;20(1):79-92.
doi: 10.1093/dnares/dss035. Epub 2012 Dec 17.

Construction of an Integrated High Density Simple Sequence Repeat Linkage Map in Cultivated Strawberry (Fragaria × Ananassa) and Its Applicability

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Free PMC article

Construction of an Integrated High Density Simple Sequence Repeat Linkage Map in Cultivated Strawberry (Fragaria × Ananassa) and Its Applicability

Sachiko N Isobe et al. DNA Res. .
Free PMC article

Abstract

The cultivated strawberry (Fragaria × ananassa) is an octoploid (2n = 8x = 56) of the Rosaceae family whose genomic architecture is still controversial. Several recent studies support the AAA'A'BBB'B' model, but its complexity has hindered genetic and genomic analysis of this important crop. To overcome this difficulty and to assist genome-wide analysis of F. × ananassa, we constructed an integrated linkage map by organizing a total of 4474 of simple sequence repeat (SSR) markers collected from published Fragaria sequences, including 3746 SSR markers [Fragaria vesca expressed sequence tag (EST)-derived SSR markers] derived from F. vesca ESTs, 603 markers (F. × ananassa EST-derived SSR markers) from F. × ananassa ESTs, and 125 markers (F. × ananassa transcriptome-derived SSR markers) from F. × ananassa transcripts. Along with the previously published SSR markers, these markers were mapped onto five parent-specific linkage maps derived from three mapping populations, which were then assembled into an integrated linkage map. The constructed map consists of 1856 loci in 28 linkage groups (LGs) that total 2364.1 cM in length. Macrosynteny at the chromosome level was observed between the LGs of F. × ananassa and the genome of F. vesca. Variety distinction on 129 F. × ananassa lines was demonstrated using 45 selected SSR markers.

Figures

Figure 1.
Figure 1.
Graphical view of syntenic relationship between F. × ananassa and F. vesca. Red and blue bars show LGs of F. × ananassa and chromosomes of F. vesca, respectively. Syntenic regions between the two species are connected by coloured lines.

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References

    1. FAOSTAT. http://faostat3.fao.org .
    1. UPOV. http://www.upov.int .
    1. Darrow G. The Strawberry. History, Breeding and Physiology. New York, USA: Holt, Rinehart and Winston; 1966.
    1. Federova N.J. Cross ability and phylogenetic relationships in the main European species of Fragaria. Natl. Acad. Sci. USSR. 1946;52:545–7.
    1. Senanayake Y.D.A., Bringhurst R.S. Origin of the Fragaria polyploids. I. Cytological evidence. Am. J. Bot. 1967;54:221–8.

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