Translational Control of Inducible Nitric Oxide Synthase by p38 MAPK in Islet β-cells

Mol Endocrinol. 2013 Feb;27(2):336-49. doi: 10.1210/me.2012-1230. Epub 2012 Dec 18.

Abstract

The MAPKs are transducers of extracellular signals such as proinflammatory cytokines. In islet β-cells, cytokines acutely activate expression of the Nos2 gene encoding inducible nitric oxide synthase (iNOS), which ultimately impairs insulin release. Because iNOS production can also be regulated posttranscriptionally, we asked whether MAPKs participate in posttranscriptional regulatory events in β-cells and primary islets in response to cytokine signaling. We show that cytokines acutely reduce cellular oxygen consumption rate and impair aconitase activity. Inhibition of iNOS with l-NMMA or inhibition of Nos2 mRNA translation with GC7 [an inhibitor of eukaryotic translation initiation factor 5A (eIF5A) activity] reversed these defects, as did inhibition of p38 MAPK by PD169316. Although inhibition of p38 had no effect on the nuclear translocation of nuclear factor κB or the abundance of Nos2 transcripts during the immediate period after cytokine exposure, its inhibition or knockdown resulted in significant reduction in iNOS protein, a finding suggestive of a permissive role for p38 in Nos2 translation. Polyribosomal profiling experiments using INS-1 β-cells revealed that Nos2 mRNA remained associated with polyribosomes in the setting of p38 inhibition, in a manner similar to that seen with blockade of translational elongation by cycloheximide. Consistent with a role in translational elongation, p38 activity is required in part for the activation of the translational factor eIF5A by promoting its hypusination. Our results suggest a novel signaling pathway in β-cells in which p38 MAPK promotes translation elongation of Nos2 mRNA via regulation of eIF5A hypusination.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aconitate Hydratase / metabolism
  • Animals
  • Cell Line
  • Cycloheximide / pharmacology
  • Cytokines / metabolism
  • Enzyme Inhibitors / pharmacology
  • Guanine / analogs & derivatives
  • Guanine / pharmacology
  • Humans
  • Imidazoles / pharmacology
  • Insulin / metabolism
  • Insulin Secretion
  • Insulin-Secreting Cells / drug effects
  • Insulin-Secreting Cells / metabolism*
  • Mice
  • Mitogen-Activated Protein Kinase 14 / antagonists & inhibitors
  • Mitogen-Activated Protein Kinase 14 / genetics
  • Mitogen-Activated Protein Kinase 14 / metabolism*
  • NF-kappa B / metabolism
  • Nitric Oxide Synthase Type II / antagonists & inhibitors
  • Nitric Oxide Synthase Type II / biosynthesis*
  • Nitric Oxide Synthase Type II / genetics*
  • Peptide Initiation Factors / antagonists & inhibitors
  • Peptide Initiation Factors / metabolism*
  • Protein Biosynthesis / drug effects
  • Protein Synthesis Inhibitors / pharmacology
  • RNA Interference
  • RNA, Small Interfering
  • RNA-Binding Proteins / antagonists & inhibitors
  • RNA-Binding Proteins / metabolism*
  • Rats
  • Rats, Wistar
  • omega-N-Methylarginine / pharmacology

Substances

  • Cytokines
  • Enzyme Inhibitors
  • Imidazoles
  • Insulin
  • N(1)-guanyl-1,7-diaminoheptane
  • NF-kappa B
  • Peptide Initiation Factors
  • Protein Synthesis Inhibitors
  • RNA, Small Interfering
  • RNA-Binding Proteins
  • eukaryotic translation initiation factor 5A
  • omega-N-Methylarginine
  • Guanine
  • Cycloheximide
  • Nitric Oxide Synthase Type II
  • Mitogen-Activated Protein Kinase 14
  • Aconitate Hydratase
  • 2-(4-nitrophenyl)-4-(4-fluorophenyl)-5-(4-pyridinyl)-1H-imidazole