Protein translocation across the rough endoplasmic reticulum

Cold Spring Harb Perspect Biol. 2013 Feb 1;5(2):a013342. doi: 10.1101/cshperspect.a013342.


The rough endoplasmic reticulum is a major site of protein biosynthesis in all eukaryotic cells, serving as the entry point for the secretory pathway and as the initial integration site for the majority of cellular integral membrane proteins. The core components of the protein translocation machinery have been identified, and high-resolution structures of the targeting components and the transport channel have been obtained. Research in this area is now focused on obtaining a better understanding of the molecular mechanism of protein translocation and membrane protein integration.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Endoplasmic Reticulum, Rough / metabolism*
  • Membrane Proteins / metabolism*
  • Membrane Transport Proteins / physiology
  • Models, Genetic
  • Models, Molecular
  • Protein Biosynthesis
  • Protein Sorting Signals
  • Protein Structure, Tertiary
  • Protein Transport / physiology
  • RNA, Messenger / metabolism


  • Membrane Proteins
  • Membrane Transport Proteins
  • Protein Sorting Signals
  • RNA, Messenger