Multinozzle emitter array chips for small-volume proteomics

Anal Chem. 2013 Jan 15;85(2):816-9. doi: 10.1021/ac3032965. Epub 2012 Dec 21.

Abstract

High-throughput multiplexed proteomics of small-volume biospecimens will generate new opportunities in theranostics. Achieving parallel top-down and bottom-up mass spectrometry analyses of target proteins using a unified apparatus will improve proteome characterization. We have developed a novel silicon-based microfluidic device, multinozzle emitter array chip (MEA chip), as a new platform for small-volume proteomics using liquid chromatography-nanoelectrospray ionization mass spectrometry (LC-nanoESI-MS). We demonstrate parallel, on-chip, and online LC-MS analysis of hemoglobin and its tryptic digests directly from microliters of blood, achieving a detection limit of less than 5 red blood cells. Our MEA chip will enable clinical proteomics of small-volume samples.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chromatography, Liquid
  • Hemoglobins / analysis*
  • Humans
  • Microfluidic Analytical Techniques* / instrumentation
  • Proteomics*
  • Silicon / chemistry
  • Spectrometry, Mass, Electrospray Ionization

Substances

  • Hemoglobins
  • Silicon