Mobility of green fluorescent protein in hydrogel-based drug-delivery systems studied by anisotropy and fluorescence recovery after photobleaching

Macromol Biosci. 2013 Feb;13(2):215-26. doi: 10.1002/mabi.201200325. Epub 2012 Dec 17.


Modified hydroxyethyl starch is photo-crosslinked in the presence of a green fluorescent protein (GFP) (mTagGFP) to obtain loaded hydrogels as model for a drug-delivery system. An important factor for the protein release is the crosslinking density since a dense network should lead to hindered diffusion. To obtain information on the rotational and translational diffusion of GFP in the hydrogel, mTagGFP is analyzed by fluorescence anisotropy and fluorescence recovery after photo-bleaching experiments using two-photon excitation. The mTagGFP shows a viscosity-retarded rotational and strongly hindered translational diffusion, depending on the polymer concentration. A comparison of anisotropy studies with mTagGFP-loaded microparticles and hydrogel disks allows the polymer concentration to be determined for the microparticles, which has been previously unknown.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anisotropy
  • Drug Delivery Systems / methods*
  • Fluorescence Polarization
  • Fluorescence Recovery After Photobleaching
  • Green Fluorescent Proteins / pharmacokinetics*
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemical synthesis
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
  • Hydroxyethyl Starch Derivatives / chemistry
  • Phosphinic Acids / chemistry
  • Propane / analogs & derivatives
  • Propane / chemistry


  • 2-hydroxy-1-(4-(hydroxyethoxy)phenyl)-2-methyl-1-propanone
  • Hydroxyethyl Starch Derivatives
  • Phosphinic Acids
  • phenyl-2,4,6-trimethylbenzoylphosphinate
  • Green Fluorescent Proteins
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Propane