A systematic review was undertaken to estimate the prevalence of chlamydia (Chlamydia trachomatis) infection among those screened in community pharmacies. The uptake of screening and clients' sociodemographic and behavioural characteristics were also reviewed. Eleven papers contributed to the final review. The proportional meta-analysis showed a chlamydia positivity of 8.1% (95% confidence interval: 7.3%-8.9%). Chlamydia screening programs in community pharmacies tended to be targeted at certain client groups such as those seeking emergency contraception in pharmacies. The pharmacists were reluctant to offer chlamydia screening to potential clients. The uptake of the service was much lower, and tended not to include men and ethnic minorities.