'Goldilocks' suppressor screen identifies web of polarity regulators

Nat Cell Biol. 2013 Jan;15(1):9-10. doi: 10.1038/ncb2666.


Genome sequencing and RNAi have been powerful allies in the quest to assign function to every gene. Systematic RNAi screens identify essential genes efficiently, but are less effective with pleiotropic or redundant genes. A common trick used by geneticists to overcome this problem is to screen for genetic interactors - mutations that enhance or suppress the phenotype of a starting mutation. Now, this classic approach has been combined with the versatility of RNAi to generate an expanded gene network for cell polarity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Comment

MeSH terms

  • Animals
  • Caenorhabditis elegans / cytology*
  • Caenorhabditis elegans / genetics*
  • Cell Polarity / genetics*
  • Gene Knockdown Techniques*
  • RNA Interference*