The yeast eukaryotic translation initiation factor 2B translation initiation complex interacts with the fatty acid synthesis enzyme YBR159W and endoplasmic reticulum membranes

Mol Cell Biol. 2013 Mar;33(5):1041-56. doi: 10.1128/MCB.00811-12. Epub 2012 Dec 21.


Using affinity purifications coupled with mass spectrometry and yeast two-hybrid assays, we show the Saccharomyces cerevisiae translation initiation factor complex eukaryotic translation initiation factor 2B (eIF2B) and the very-long-chain fatty acid (VLCFA) synthesis keto-reductase enzyme YBR159W physically interact. The data show that the interaction is specifically between YBR159W and eIF2B and not between other members of the translation initiation or VLCFA pathways. A ybr159wΔ null strain has a slow-growth phenotype and a reduced translation rate but a normal GCN4 response to amino acid starvation. Although YBR159W localizes to the endoplasmic reticulum membrane, subcellular fractionation experiments show that a fraction of eIF2B cofractionates with lipid membranes in a YBR159W-independent manner. We show that a ybr159wΔ yeast strain and other strains with null mutations in the VLCFA pathway cause eIF2B to appear as numerous foci throughout the cytoplasm.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 3-Hydroxyacyl CoA Dehydrogenases / analysis
  • 3-Hydroxyacyl CoA Dehydrogenases / metabolism*
  • Endoplasmic Reticulum / metabolism
  • Eukaryotic Initiation Factor-2B / analysis
  • Eukaryotic Initiation Factor-2B / metabolism*
  • Fatty Acids / metabolism*
  • Protein Interaction Mapping
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / analysis
  • Saccharomyces cerevisiae Proteins / metabolism*


  • Eukaryotic Initiation Factor-2B
  • Fatty Acids
  • Saccharomyces cerevisiae Proteins
  • 3-Hydroxyacyl CoA Dehydrogenases
  • YBR159W protein, S cerevisiae