Impact of bone marrow-derived mesenchymal stromal cells on experimental xenogeneic graft-versus-host disease

Cytotherapy. 2013 Mar;15(3):267-79. doi: 10.1016/j.jcyt.2012.09.003. Epub 2012 Dec 21.


Background aims: Graft-versus-host disease (GVHD) is a life-threatening complication of allogeneic hematopoietic cell transplantation caused by donor T cells reacting against host tissues. Previous studies have suggested that mesenchymal stromal cells (MSCs) could exert potent immunosuppressive effects.

Methods: The ability of human bone marrow derived MSCs to prevent xenogeneic GVHD in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice and in NOD/SCID/interleukin-2Rγ(null) (NSG) mice transplanted with human peripheral blood mononuclear cells (PBMCs) was assessed.

Results: Injection of 200 × 10(6) human PBMCs intraperitoneally (IP) into sub-lethally (3.0 Gy) irradiated NOD/SCID mice also given anti-asialo GM1 antibodies IP 1 day prior and 8 days after transplantation induced lethal xenogeneic GVHD in all tested mice. Co-injection of 2 × 10(6) MSCs IP on day 0 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs. Similarly, injection of 30 × 10(6) human PBMCs IP into sub-lethally (2.5 Gy) irradiated NSG mice induced a lethal xenogeneic GVHD in all tested mice. Injection of 3 × 10(6) MSCs IP on days 0, 7, 14 and 21 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs.

Conclusions: Injection of MSCs did not prevent xenogeneic GVHD in these two humanized mice models.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology
  • Graft vs Host Disease / etiology
  • Graft vs Host Disease / genetics
  • Graft vs Host Disease / physiopathology*
  • Humans
  • Leukocytes, Mononuclear / transplantation
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology*
  • Mice
  • Mice, SCID
  • T-Lymphocytes / immunology
  • T-Lymphocytes / transplantation
  • Transplantation, Homologous / adverse effects