A rare point mutation in the Ras oncogene in hepatocellular carcinoma

Surg Today. 2013 Mar;43(3):289-92. doi: 10.1007/s00595-012-0462-8. Epub 2012 Dec 26.


Purpose: The Ras gene is one of the oncogenes most frequently detected in human cancers, and codes for three proteins (K-, N-, and H-Ras). The aim of this study was to examine the mutations in codons 12, 13 and 61 of the three Ras genes in cases of human hepatocellular carcinoma (HCC).

Methods: Paired samples of HCC and corresponding non-malignant liver tissue were collected from 61 patients who underwent hepatectomy. A dot-blot analysis was used to analyze the products of the polymerase chain reaction (PCR) amplification of codons 12, 13, and 61 of K-, N- and H-Ras for mutations.

Results: Only one mutation (K-Ras codon 13; Gly to Asp) was detected among the 61 patients. Interestingly, this patient had a medical history of surgery for both gastric cancer and right lung cancer. No mutations were found in codons 12 and 61 of K-Ras or codons 12, 13 and 61 of the N-Ras and H-Ras genes in any of the HCCs or corresponding non-malignant tissues.

Conclusions: These findings indicated that the activation of Ras proto-oncogenes by mutations in codons 12, 13, and 61 does not play a major role in hepatocellular carcinogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Carcinoma, Hepatocellular / genetics*
  • Codon
  • DNA, Neoplasm / genetics*
  • Female
  • Genes, ras / genetics*
  • Humans
  • Liver Neoplasms / genetics*
  • Male
  • Middle Aged
  • Point Mutation*
  • Polymerase Chain Reaction
  • Retrospective Studies


  • Codon
  • DNA, Neoplasm