Amino acid 316 of hemagglutinin and the neuraminidase stalk length influence virulence of H9N2 influenza virus in chickens and mice

J Virol. 2013 Mar;87(5):2963-8. doi: 10.1128/JVI.02688-12. Epub 2012 Dec 26.

Abstract

H9N2 influenza viruses with an A316S substitution in hemagglutinin (HA) and a shorter neuraminidase (NA) stalk have become predominant in China. The A316S was shown to increase HA cleavage efficiency when combined with short stalk NA, and the short stalk NA improved NA enzyme activity and release of virus from erythrocytes. Single mutations or combinations of these mutations strengthened the virulence of H9N2 virus in chickens and mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Animals
  • Chick Embryo
  • Chickens
  • Dogs
  • Erythrocytes / virology
  • Hemagglutinin Glycoproteins, Influenza Virus / chemistry*
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics*
  • Hemagglutinin Glycoproteins, Influenza Virus / metabolism
  • Influenza A Virus, H9N2 Subtype / chemistry*
  • Influenza A Virus, H9N2 Subtype / genetics
  • Influenza A Virus, H9N2 Subtype / pathogenicity*
  • Madin Darby Canine Kidney Cells
  • Mice
  • Mutation
  • Neuraminidase / chemistry*
  • Neuraminidase / genetics*
  • Neuraminidase / metabolism
  • Orthomyxoviridae Infections / virology*
  • Poultry Diseases / virology
  • Virus Release

Substances

  • Hemagglutinin Glycoproteins, Influenza Virus
  • Neuraminidase