Pioneer glutamatergic cells develop into a morpho-functionally distinct population in the juvenile CA3 hippocampus

Nat Commun. 2012:3:1316. doi: 10.1038/ncomms2318.

Abstract

The developing CA3 hippocampus is comprised by highly connected hub neurons that are particularly effective in achieving network synchronization. Functional hub neurons were shown to be exclusively GABAergic, suggesting that the contribution of glutamatergic neurons to physiological synchronization processes at early postnatal stages is minimal. However, without fast GABAergic transmission, a different situation may prevail. In the adult CA3, blocking fast GABAergic transmission induces the generation of network bursts that can be triggered by the stimulation of single pyramidal neurons. Here we revisit the network function of CA3 glutamatergic neurons from a developmental viewpoint, without fast GABAergic transmission. We uncover a sub-population of early-generated glutamatergic neurons that impacts network dynamics when stimulated in the juvenile hippocampus. Additionally, this population displays characteristic morpho-physiological features in the juvenile and adult hippocampus. Therefore, the apparently homogeneous glutamatergic cell population likely displays a morpho-functional diversity rooted in temporal embryonic origins.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CA3 Region, Hippocampal / cytology*
  • CA3 Region, Hippocampal / embryology
  • CA3 Region, Hippocampal / growth & development
  • CA3 Region, Hippocampal / metabolism*
  • Female
  • Glutamic Acid / metabolism*
  • Male
  • Mice / embryology
  • Mice / genetics
  • Mice / growth & development
  • Mice / metabolism
  • Mice, Transgenic
  • Neurogenesis
  • Neurons / cytology*
  • Neurons / metabolism
  • gamma-Aminobutyric Acid / metabolism

Substances

  • Glutamic Acid
  • gamma-Aminobutyric Acid