Regulation of microtubule stability and organization by mammalian Par3 in specifying neuronal polarity

Dev Cell. 2013 Jan 14;24(1):26-40. doi: 10.1016/j.devcel.2012.11.014. Epub 2012 Dec 27.


Polarization of mammalian neurons with a specified axon requires precise regulation of microtubule and actin dynamics in the developing neurites. Here we show that mammalian partition defective 3 (mPar3), a key component of the Par polarity complex that regulates the polarization of many cell types including neurons, directly regulates microtubule stability and organization. The N-terminal portion of mPar3 exhibits strong microtubule binding, bundling, and stabilization activity, which can be suppressed by its C-terminal portion via an intramolecular interaction. Interestingly, the intermolecular oligomerization of mPar3 is able to relieve the intramolecular interaction and thereby promote microtubule bundling and stabilization. Furthermore, disruption of this microtubule regulatory activity of mPar3 impairs its function in axon specification. Together, these results demonstrate a role for mPar3 in directly regulating microtubule organization that is crucial for neuronal polarization.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Axons / metabolism
  • COS Cells
  • Cell Adhesion Molecules / antagonists & inhibitors
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism*
  • Cell Polarity*
  • Cells, Cultured
  • Cytoskeleton / metabolism
  • Fluorescence Resonance Energy Transfer
  • Green Fluorescent Proteins
  • Hippocampus / cytology
  • Hippocampus / metabolism
  • Immunoprecipitation
  • Mice
  • Microtubules / chemistry
  • Microtubules / physiology*
  • Molecular Sequence Data
  • Neurogenesis / physiology*
  • Neurons / cytology*
  • Neurons / metabolism
  • Protein Structure, Tertiary
  • RNA, Small Interfering / genetics
  • Sequence Homology, Amino Acid
  • Signal Transduction


  • Cell Adhesion Molecules
  • Pard3 protein, mouse
  • RNA, Small Interfering
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins