Zinc sulphate and vitamin E alleviate reproductive toxicity caused by aluminium sulphate in male albino rats

Toxicol Ind Health. 2015 Mar;31(3):221-34. doi: 10.1177/0748233712469650. Epub 2013 Jan 2.


This study was designed to investigate the reproductive toxicity of aluminium sulphate and the therapeutic effects of administration of zinc sulphate and vitamin E individually or in combination against the toxic effect caused by aluminium (Al) in male albino rats. The animals were divided into five groups: group 1 received distilled water and served as control; group 2 received only aluminium sulphate (50 mg/kg body weight (b.w.)); group 3 received aluminium sulphate (50 mg/kg b.w.) plus zinc sulphate (50 mg/kg b.w.); group 4 received aluminium sulphate (50 mg/kg b.w.) and vitamin E (15 mg/kg b.w.); group 5 received aluminium sulphate plus a combination of zinc sulphate and vitamin E in similar doses as above. Doses were administered orally once daily for 45 consecutive days. The results revealed that aluminium sulphate induced significant decrease in body weight gain and testis weight and significant increase in Al level in both serum and testes of male rats. Biochemical analysis showed significant decrease in serum total protein and phospholipids levels, while serum total lipid was significantly elevated post Al treatment. In addition, significant decrease in total protein, phospholipids and cholesterol levels in the testes of Al-treated rats was recorded. The data also showed significant decrease in the levels of serum testosterone, leutinizing hormone and follicle stimulating hormone and significant increase in the level of serum prolactin in Al-intoxicated rats. Moreover, histological examination showed that aluminium sulphate caused apparent alterations in the testicular structure of the treated animals. Treatment with zinc sulphate and vitamin E individually or in combination ameliorated the harmful effects of Al, which was proved histopathologically by the noticeable improvement in the testicular tissues. We can conclude that the tested dose of aluminium sulphate induced toxic effect on the reproductive system of male albino rats and the treatment with zinc sulphate and/or vitamin E alleviated these toxic effects. In some cases, vitamin E exerted a more potent effect, while in other cases, the more potent effect is related to zinc sulphate and the combination of both at most of the recorded data.

Keywords: Aluminium sulphate; male albino rats; reproductive toxicity; vitamin E; zinc sulphate.

MeSH terms

  • Administration, Oral
  • Alum Compounds / toxicity*
  • Animals
  • Antioxidants / administration & dosage
  • Disease Models, Animal*
  • Dose-Response Relationship, Drug
  • Drug Administration Schedule
  • Drug Therapy, Combination
  • Follicle Stimulating Hormone / blood
  • Luteinizing Hormone / blood
  • Male
  • Organ Size / drug effects
  • Prolactin / blood
  • Protective Agents / administration & dosage*
  • Rats
  • Testis / drug effects*
  • Testis / pathology*
  • Testosterone / blood
  • Vitamin E / administration & dosage*
  • Zinc Sulfate / administration & dosage*


  • Alum Compounds
  • Antioxidants
  • Protective Agents
  • Vitamin E
  • aluminum sulfate
  • Testosterone
  • Zinc Sulfate
  • Prolactin
  • Luteinizing Hormone
  • Follicle Stimulating Hormone