The Caenorhabditis elegans THO complex is required for the mitotic cell cycle and development

PLoS One. 2012;7(12):e52447. doi: 10.1371/journal.pone.0052447. Epub 2012 Dec 20.

Abstract

THO is a conserved eukaryotic complex involved in mRNP biogenesis and RNA export that plays an important role in preventing transcription- and RNA-mediated genome instability in mitosis and meiosis. In mammals THO is essential for embryogenesis, which limits our capacity to analyze the physiological relevance of THO during development and in adult organisms. Using Caenorhabditis elegans as a model system we show that the THO complex is essential for mitotic genome integrity and the developmentally regulated mitotic cell cycles occurring during late postembryonic stages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Size
  • Caenorhabditis elegans / cytology*
  • Caenorhabditis elegans / growth & development
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Cell Cycle Checkpoints
  • DNA Damage
  • DNA Replication
  • Mitosis*
  • Multiprotein Complexes / metabolism*
  • Mutation / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • Multiprotein Complexes
  • THOC-2 protein, C elegans
  • Transcription Factors

Grant support

This work was supported by grants from the Spanish Ministry of Science and Innovation (BFU2010-16372 and Consolider Ingenio 2010 CSD2007-0015), the European Union (FEDER) and the Junta de Andalucía (CVI4567). MCP was the recipient of a predoctoral training fellowship from the Spanish National Institute of Health Carlos III. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.