Abstract
We investigated the effects of equol on adipogenesis by measuring lipid accumulation and analyzing the change in adipocyte-related gene expression in MC3T3-L1 cells. Treatment with 10 µM equol tended to increase adipocyte-related gene expression, whereas 100 µM equol reduced lipid accumulation and suppressed the expression of these genes and proteins. Our results suggest that equol regulated adipogenesis in a bi-phasic fashion.
MeSH terms
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3T3-L1 Cells
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Adipocytes / cytology
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Adipocytes / drug effects*
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Adipocytes / metabolism
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Adipogenesis / drug effects*
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Adipogenesis / genetics
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Animals
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CCAAT-Enhancer-Binding Proteins / genetics
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CCAAT-Enhancer-Binding Proteins / metabolism
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CD36 Antigens / genetics
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CD36 Antigens / metabolism
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Cell Differentiation / drug effects
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Cell Proliferation / drug effects
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Dose-Response Relationship, Drug
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Equol / pharmacology*
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Gene Expression Regulation / drug effects
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Lipid Metabolism / drug effects*
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Mice
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PPAR gamma / genetics
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PPAR gamma / metabolism
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Phytoestrogens / pharmacology*
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fas Receptor / genetics
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fas Receptor / metabolism
Substances
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CCAAT-Enhancer-Binding Proteins
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CD36 Antigens
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CEBPA protein, mouse
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Fas protein, mouse
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PPAR gamma
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Phytoestrogens
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fas Receptor
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Equol