Bi-phasic effect of equol on adipocyte differentiation of MC3T3-L1 cells

Biosci Biotechnol Biochem. 2013;77(1):201-4. doi: 10.1271/bbb.120677. Epub 2013 Jan 7.

Abstract

We investigated the effects of equol on adipogenesis by measuring lipid accumulation and analyzing the change in adipocyte-related gene expression in MC3T3-L1 cells. Treatment with 10 µM equol tended to increase adipocyte-related gene expression, whereas 100 µM equol reduced lipid accumulation and suppressed the expression of these genes and proteins. Our results suggest that equol regulated adipogenesis in a bi-phasic fashion.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology
  • Adipocytes / drug effects*
  • Adipocytes / metabolism
  • Adipogenesis / drug effects*
  • Adipogenesis / genetics
  • Animals
  • CCAAT-Enhancer-Binding Proteins / genetics
  • CCAAT-Enhancer-Binding Proteins / metabolism
  • CD36 Antigens / genetics
  • CD36 Antigens / metabolism
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Equol / pharmacology*
  • Gene Expression Regulation / drug effects
  • Lipid Metabolism / drug effects*
  • Mice
  • PPAR gamma / genetics
  • PPAR gamma / metabolism
  • Phytoestrogens / pharmacology*
  • fas Receptor / genetics
  • fas Receptor / metabolism

Substances

  • CCAAT-Enhancer-Binding Proteins
  • CD36 Antigens
  • CEBPA protein, mouse
  • Fas protein, mouse
  • PPAR gamma
  • Phytoestrogens
  • fas Receptor
  • Equol