Study on noninvasive laboratory tests for fibrosis in chronic HBV infection and their evaluation

J Clin Lab Anal. 2013 Jan;27(1):5-11. doi: 10.1002/jcla.21554. Epub 2013 Jan 4.


Background: Noninvasive laboratory tests have been widely used in the diagnosis of liver fibrosis. This study was designed to evaluate the diagnostic value of the four serum markers detected on a JETLIA-962 chemiluminescence analyzer and the FibroTest index for chronic hepatitis B (CHB) related liver fibrosis.

Methods: A JETLIA-962 chemiluminescence analyzer was used to measure the hyaluronic acid (HA), laminin (LN), procollagen III N-terminal peptide (PIIINP), and type IV collagen (CIV) contents in the sera of CHB patients and controls.

Results: In our research, it was found that serum HA, LN, PIIINP, and CIV concentrations and the FibroTest index were correlated with fibrosis stage. The FibroTest index had the largest area under the receiver operating characteristic (ROC) curve for fibrosis and cirrhosis (0.80 and 0.776, respectively). The five indices together were able to exclude fibrosis with a negative likelihood ratio <0.1. The logistic regression equations for diagnosing liver fibrosis and early cirrhosis were separately established.

Conclusions: We found that the JETLIA-962 chemiluminescence analyzer was of great value in diagnosis of liver fibrosis. Combining five indices improved the diagnostic efficiency and reduced the incidences of unnecessary liver biopsies. Two logistic regression equations established may be helpful in diagnosing liver fibrosis and early cirrhosis, which need to be further evaluated.

MeSH terms

  • Adolescent
  • Adult
  • Biomarkers / blood
  • Case-Control Studies
  • Collagen Type IV / blood
  • Female
  • Hepatitis B, Chronic / blood*
  • Hepatitis B, Chronic / pathology*
  • Humans
  • Hyaluronic Acid / blood
  • Laminin / blood
  • Liver Cirrhosis / blood*
  • Liver Cirrhosis / pathology
  • Liver Cirrhosis / virology*
  • Logistic Models
  • Male
  • Middle Aged
  • Peptide Fragments / blood
  • Procollagen / blood
  • ROC Curve


  • Biomarkers
  • Collagen Type IV
  • Laminin
  • Peptide Fragments
  • Procollagen
  • procollagen Type III-N-terminal peptide
  • Hyaluronic Acid