Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Sep;19(3):279-86.
doi: 10.4103/0971-6580.103669.

In Vivo Comparative Studies on Antigenotoxicity of Date Palm (Phoenix Dactylifera L.) Pits Extract Against DNA Damage Induced by N-Nitroso-N-methylurea in Mice

Affiliations
Free PMC article

In Vivo Comparative Studies on Antigenotoxicity of Date Palm (Phoenix Dactylifera L.) Pits Extract Against DNA Damage Induced by N-Nitroso-N-methylurea in Mice

K A S Diab et al. Toxicol Int. .
Free PMC article

Abstract

Aqueous extract of the date palm (Phoenix dactylifera L.) pits was prepared and its antigenotoxic activity was evaluated against N-Nitroso-N-methylurea (NMU) induced mutagenic effect in mice, using chromosome aberration (CA), micronuclei (MN) and DNA fragmentation assays as experimental end points in male mice. Date pits extract (DPE) was given orally to mice at the dose 25 mg/25 g mouse for successive five days in a week up to four consecutive weeks. NMU was used as mutagen and was given intraperitoneal (i.p) injection at single dose 80 mg/kg b.w., 24 hr after last dose of DPE in pre-treatment regimen and 24 hr before the first dose of DPE in the post-treatment regimen. Mice were scarified after one, two and seven days after the end of treatment. The results have shown that pre-and post-treatment regimens of DPE were significantly restored the DNA damage induced by NMU, as revealed by lowering of the occurrence of CAs and MN in bone marrow cells and inhibition of hepatic DNA fragmentation. These findings suggested that DPE produced their inhibitory activity either by desmutagenic or bioantimutagenic manner in pre-and post-treatment regimens respectively.

Keywords: Chromosome aberration assay; DNA fragmentation assay; N-Nitro-N-methylurea; date palm pits; micronucleus assay.

Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Metaphases plates from mouse bone marrow cells after i.p. treatment with NMU showing a) break, b) Aneuploidy with ring chromosome.
Figure 2
Figure 2
Difference in size of micronuclei from mouse bone marrow cells after i.p. treatment with NMU

Similar articles

See all similar articles

Cited by 3 articles

References

    1. Soong Y, Barlow PJ. Antioxidant activity and phenolic content of selected fruit seeds. Food Chem. 2004;88:411–7.
    1. Part III: Plant Production, Statistics Division. Vol. 28. Arab Organization for Agricultural Development: ANS Publisher; 2008. Arab Organization for Agricultural Development (AOAD). Arab Agricultural Statistics Yearbook.
    1. El-Juhany LI. Degradation of date palm trees and date production in Arab countries: causes and potential rehabilitation. Aust J Basic and Appl Sci. 2010;4:3998–4010.
    1. Al-Shahib W, Marshall RJ. The fruit of the date palm: Its possible use as the best food for the future. Int J Food Sci Nutr. 2003;54:247–59. - PubMed
    1. Baliga MS, Baligab BRV, Kandathilc SM, Bhatd HP, Vayalile PK. A review of the chemistry and pharmacology of the date fruits (Phoenix dactylifera L.) Food Res Inter. 2011;44:1812–22.

LinkOut - more resources

Feedback