Monitoring nutrient signaling through the longevity protein p66(SHC¹)

Sofia Chiatamone Ranieri; Giovambattista Pani

doi:10.1007/978-1-62703-239-1_23

Monitoring nutrient signaling through the longevity protein p66(SHC¹)

Methods Mol Biol. 2013:965:341-53. doi: 10.1007/978-1-62703-239-1_23.

Authors

Sofia Chiatamone Ranieri¹, Giovambattista Pani

Affiliation

¹ Clinical Chemistry, Laboratory and Endocrinology Unit, Departement of Laboratory Medicine, Azienda Ospedaliera ASMN, Istituto di Ricovero e Cura a Carattere Scientifico, Reggio Emilia, Italy.

PMID: 23296670
DOI: 10.1007/978-1-62703-239-1_23

Abstract

Nutrient availability and nutrient-dependent biochemical signals represent major determinants of cellular senescence and organismal aging. The present chapter describes simple procedures to reliably evaluate the response of cultured cell to nutrients through the longevity protein p66(SHC1) and the mTOR/S6K cascade, which might be used to study cellular senescence and its chemical modulation by pharmaceutical agents in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aging
Electrophoresis
Gene Knockdown Techniques
HEK293 Cells
Humans
Longevity*
Phosphorylation
Shc Signaling Adaptor Proteins / deficiency
Shc Signaling Adaptor Proteins / genetics
Shc Signaling Adaptor Proteins / isolation & purification
Shc Signaling Adaptor Proteins / metabolism*
Signal Transduction*
Src Homology 2 Domain-Containing, Transforming Protein 1
Staining and Labeling
Transfection

Substances

SHC1 protein, human
Shc Signaling Adaptor Proteins
Src Homology 2 Domain-Containing, Transforming Protein 1