Assessing chronological aging in Saccharomyces cerevisiae

Methods Mol Biol. 2013;965:463-72. doi: 10.1007/978-1-62703-239-1_30.


Saccharomyces cerevisiae is one of the most studied model organisms for the identification of genes and mechanisms that affect aging. The chronological lifespan (CLS) assay, which monitors the survival of a non-dividing population, is one of the two methods to study aging in yeast. To eliminate potential artifacts and identify genes and signaling pathways that may also affect aging in higher eukaryotes, it is important to determine CLS by multiple methods. Here, we describe these methods as well as the assays to study macromolecular damage during aging in yeast, with a focus on genomic instability.

MeSH terms

  • Cell Nucleus / genetics
  • Culture Media / chemistry
  • DNA, Fungal / genetics
  • Genomic Instability
  • Mutation Rate
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development*
  • Time Factors
  • Water / chemistry


  • Culture Media
  • DNA, Fungal
  • Water