Functional genomics identifies type I interferon pathway as central for host defense against Candida albicans

Nat Commun. 2013;4:1342. doi: 10.1038/ncomms2343.

Abstract

Candida albicans is the most common human fungal pathogen causing mucosal and systemic infections. However, human antifungal immunity remains poorly defined. Here by integrating transcriptional analysis and functional genomics, we identified Candida-specific host defence mechanisms in humans. Candida induced significant expression of genes from the type I interferon pathway in human peripheral blood mononuclear cells. This unexpectedly prominent role of type I interferon pathway in anti-Candida host defence was supported by additional evidence. Polymorphisms in type I interferon genes modulated Candida-induced cytokine production and were correlated with susceptibility to systemic candidiasis. In in vitro experiments, type I interferons skewed Candida-induced inflammation from a Th17 response towards a Th1 response. Patients with chronic mucocutaneous candidiasis displayed defective expression of genes in the type I interferon pathway. These findings indicate that the type I interferon pathway is a main signature of Candida-induced inflammation and has a crucial role in anti-Candida host defence in humans.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / immunology*
  • Candidemia / genetics
  • Candidemia / immunology
  • Candidemia / microbiology
  • Candidiasis, Chronic Mucocutaneous / genetics
  • Candidiasis, Chronic Mucocutaneous / immunology
  • Candidiasis, Chronic Mucocutaneous / microbiology
  • Case-Control Studies
  • Cluster Analysis
  • Gene Expression Regulation
  • Genetic Predisposition to Disease
  • Genomics*
  • Host-Pathogen Interactions / genetics*
  • Host-Pathogen Interactions / immunology*
  • Humans
  • Interferon Type I / genetics*
  • Interferon Type I / immunology*
  • Mutation / genetics
  • Polymorphism, Single Nucleotide / genetics
  • STAT1 Transcription Factor / genetics
  • Signal Transduction / genetics*
  • Signal Transduction / immunology
  • Th1 Cells / immunology
  • Th17 Cells / immunology
  • Transcription, Genetic

Substances

  • Interferon Type I
  • STAT1 Transcription Factor

Associated data

  • GEO/GSE42606
  • GEO/GSE42630