Nucleolar Transcription Factor hUBF Contains a DNA-binding Motif With Homology to HMG Proteins

Nature. 1990 Apr 26;344(6269):830-6. doi: 10.1038/344830a0.

Abstract

The eukaryotic upstream binding factor (UBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor, SL1. Isolation of complementary DNA clones and sequence analysis reveals similarities between DNA binding domains of human UBF (hUBF) and high mobility group (HMG) protein 1. Expression, cellular localization and in vitro transcription studies establish that cloned hUBF encodes a nucleolar factor that binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • Cell Nucleolus / analysis*
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • DNA-Binding Proteins* / genetics
  • High Mobility Group Proteins*
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Mutation
  • Pol1 Transcription Initiation Complex Proteins*
  • Promoter Regions, Genetic
  • RNA Polymerase I / metabolism
  • RNA, Ribosomal / genetics
  • Sequence Homology, Nucleic Acid
  • Transcription Factors* / genetics
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • High Mobility Group Proteins
  • Pol1 Transcription Initiation Complex Proteins
  • RNA, Ribosomal
  • Transcription Factors
  • transcription factor UBF
  • DNA
  • RNA Polymerase I

Associated data

  • GENBANK/X53461