Probing membrane protein interactions with their lipid raft environment using single-molecule tracking and Bayesian inference analysis

PLoS One. 2013;8(1):e53073. doi: 10.1371/journal.pone.0053073. Epub 2013 Jan 3.

Abstract

The statistical properties of membrane protein random walks reveal information on the interactions between the proteins and their environments. These interactions can be included in an overdamped Langevin equation framework where they are injected in either or both the friction field and the potential field. Using a Bayesian inference scheme, both the friction and potential fields acting on the ε-toxin receptor in its lipid raft have been measured. Two types of events were used to probe these interactions. First, active events, the removal of cholesterol and sphingolipid molecules, were used to measure the time evolution of confining potentials and diffusion fields. Second, passive rare events, de-confinement of the receptors from one raft and transition to an adjacent one, were used to measure hopping energies. Lipid interactions with the ε-toxin receptor are found to be an essential source of confinement. ε-toxin receptor confinement is due to both the friction and potential field induced by cholesterol and sphingolipids. Finally, the statistics of hopping energies reveal sub-structures of potentials in the rafts, characterized by small hopping energies, and the difference of solubilization energy between the inner and outer raft area, characterized by higher hopping energies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Animals
  • Bacterial Toxins / chemistry
  • Bayes Theorem
  • Cell Line
  • Cholesterol / chemistry
  • Clostridium perfringens
  • Dogs
  • Lipids / chemistry*
  • Madin Darby Canine Kidney Cells
  • Membrane Microdomains / chemistry*
  • Membrane Proteins / chemistry*
  • Models, Statistical
  • Nanoparticles / chemistry
  • Protein Interaction Mapping*
  • Receptors, Transferrin / chemistry
  • Sphingolipids / chemistry
  • Time Factors

Substances

  • Bacterial Toxins
  • Lipids
  • Membrane Proteins
  • Receptors, Transferrin
  • Sphingolipids
  • Cholesterol

Grants and funding

This work was supported by the Délégation Générale pour l'Armement (DGA), Centre de competences en Nanosciences C'Nano Ile-de-France, Fondation de France, ANR Piribio, Bayer Science and Education Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.