Genome-wide identification of Ikaros targets elucidates its contribution to mouse B-cell lineage specification and pre-B-cell differentiation

Blood. 2013 Mar 7;121(10):1769-82. doi: 10.1182/blood-2012-08-450114. Epub 2013 Jan 9.


Ikaros family DNA-binding proteins are critical regulators of B-cell development. Because the current knowledge of Ikaros targets in B-cell progenitors is limited, we have identified genes that are bound and regulated by Ikaros in pre-B cells. To elucidate the role of Ikaros in B-cell lineage specification and differentiation, we analyzed the differential expression of Ikaros targets during the progression of multipotent to lymphoid-restricted progenitors, B- and T-cell lineage specification, and progression along the B-cell lineage. Ikaros targets accounted for one-half of all genes up-regulated during B-cell lineage specification in vivo, explaining the essential role of Ikaros in this process. Expression of the Ikaros paralogs Ikzf1 and Ikzf3 increases incrementally during B-cell progenitor differentiation, and, remarkably, inducible Ikaros expression in cycling pre-B cells was sufficient to drive transcriptional changes resembling the differentiation of cycling to resting pre-Bcells in vivo. The data suggest that Ikaros transcription factor dosage drives the progression of progenitors along a predetermined lineage by regulating multiple targets in key pathways, including pre-B–cell receptor signaling, cell cycle progression, and lymphocyte receptor rearrangement.Our approachmay be of general use to map the contribution of transcription factors to cell lineage commitment and differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / metabolism
  • Binding Sites
  • Cell Cycle
  • Cell Differentiation*
  • Cell Lineage*
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Gene Expression Regulation
  • Genome*
  • Ikaros Transcription Factor / genetics
  • Ikaros Transcription Factor / metabolism*
  • Lymphocyte Activation
  • Mice
  • Precursor Cells, B-Lymphoid / cytology*
  • Precursor Cells, B-Lymphoid / metabolism
  • Signal Transduction
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*


  • Transcription Factors
  • Ikaros Transcription Factor

Associated data

  • GEO/GSE38110
  • GEO/GSE38169
  • GEO/GSE42462
  • GEO/GSE42551