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. 2012:2012:846052.
doi: 10.1155/2012/846052. Epub 2012 Dec 6.

Association between polymorphisms in interleukin-17A and -17F genes and chronic periodontal disease

Jôice Dias Corrêa  1 , Mila Fernandes Moreira Madeira, Renata Gonçalves Resende, Jeane de Fátima Correia-Silva, Ricardo Santiago Gomez, Danielle da Glória de Souza, Mauro Martins Teixeira, Celso Martins Queiroz-Junior, Tarcília Aparecida da Silva
Affiliations

Association between polymorphisms in interleukin-17A and -17F genes and chronic periodontal disease

Jôice Dias Corrêa et al. Mediators Inflamm. 2012.

Abstract

Objective: Interleukin-17 (IL-17) is a cytokine that induces neutrophil recruitment and the release of inflammatory mediators in several inflammatory conditions; nevertheless, the involvement of IL-17 gene polymorphisms in chronic periodontitis (CP) has not been addressed yet. Our aim was to evaluate the association between periodontal status and the polymorphisms IL-17A G197A and IL-17F C7488T in subjects with CP along with their impact on levels of inflammatory mediators.

Material and methods: Genomic DNA was obtained from 30 CP patients and 30 healthy controls (HCs). IL-17A G197A and IL-17F C7488T polymorphisms were determined using PCR-RFLP. Serum and periodontal tissues were collected and processed for ELISA, myeloperoxidase (MPO), and/or microscopic analysis.

Results: The frequencies of genotypes in the CP group were significantly different from those of HC. Odds ratio indicated that increased risks for CP were associated with the -197A allele, not with the -7488T allele. In addition, the -197A allele was correlated with worse clinical parameters, higher MPO activity, and increased expression of inflammatory mediators (IL-17A and IL-8) than the other genotypes.

Conclusions: These results indicate that the IL-17A -197A allele is associated with increased risk for CP, likely because this genotype relates to the enhanced inflammation in periodontal tissues.

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Figures

Figure 1
Figure 1
Levels of (a) IL-17A and (b) IL-17F in the gingival tissue and serum samples from CP and HC subjects. (c) Levels of IL-8 and (d) MPO activity in gingival tissue samples from CP and HC subjects. *Statistically significant difference at P < 0.05. HC: healthy control, CP: chronic periodontitis, and MPO: myeloperoxidase.
Figure 2
Figure 2
Levels of inflammatory mediators in chronic periodontitis patients according to each IL-17A G197A genotype. (a) Levels of IL-17A in gingival tissues and (b) serum. (c) Levels of IL-8 and (d) MPO (myeloperoxidase) activity in gingival tissue samples. *Statistically significant difference (P < 0.05) comparing with the genotype GG.
Figure 3
Figure 3
Levels of inflammatory mediators in chronic periodontitis patients according to each IL-17F C7488T genotype. (a) Levels of IL-17F in gingival tissues and (b) serum. (c) Levels of IL-8 and (d) MPO (myeloperoxidase) activity in gingival tissue samples.
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