2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

J Biol Eng. 2013 Jan 10;7(1):2. doi: 10.1186/1754-1611-7-2.


There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.