Characterization and cellular interaction of fluorescent-labeled PHEMA nanoparticles

Artif Cells Nanomed Biotechnol. 2013 Apr;41(2):78-84. doi: 10.3109/21691401.2012.742099. Epub 2013 Jan 10.

Abstract

The aim of this study is to label the cells with polymeric nanoparticles properly and efficiently. For this purpose, acridine orange (AO)-loaded poly(2-hydroxyethyl methacrylate) (PHEMA) nanoparticles were synthesized by miniemulsion polymerization. PHEMA nanoparticles were characterized by zeta sizer, zeta potential, atomic force microscopy (AFM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), fluorescence microscopy, Fourier transform infrared spectrophotometer (FTIR), and elemental analysis. In addition, the toxicity of the nanoparticles were investigated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay.

MeSH terms

  • Acridine Orange
  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Drug Carriers / chemical synthesis*
  • Drug Carriers / pharmacology
  • Emulsions
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Fluorescent Dyes
  • Mice
  • Microscopy, Atomic Force
  • Microscopy, Electron, Transmission
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Particle Size
  • Polyhydroxyethyl Methacrylate / chemistry*
  • Polymerization
  • Spectroscopy, Fourier Transform Infrared
  • Staining and Labeling
  • Tetrazolium Salts
  • Thiazoles

Substances

  • Drug Carriers
  • Emulsions
  • Fluorescent Dyes
  • Tetrazolium Salts
  • Thiazoles
  • Polyhydroxyethyl Methacrylate
  • thiazolyl blue
  • Acridine Orange