Cough aerosols of Mycobacterium tuberculosis predict new infection: a household contact study

Am J Respir Crit Care Med. 2013 May 1;187(9):1007-15. doi: 10.1164/rccm.201208-1422OC.


Rationale: Airborne transmission of Mycobacterium tuberculosis results from incompletely characterized host, bacterial, and environmental factors. Sputum smear microscopy is associated with considerable variability in transmission.

Objectives: To evaluate the use of cough-generated aerosols of M. tuberculosis to predict recent transmission.

Methods: Patients with pulmonary tuberculosis (TB) underwent a standard evaluation and collection of cough aerosol cultures of M. tuberculosis. We assessed household contacts for new M. tuberculosis infection. We used multivariable logistic regression analysis with cluster adjustment to analyze predictors of new infection.

Measurements and main results: From May 2009 to January 2011, we enrolled 96 sputum culture-positive index TB cases and their 442 contacts. Only 43 (45%) patients with TB yielded M. tuberculosis in aerosols. Contacts of patients with TB who produced high aerosols (≥10 CFU) were more likely to have a new infection compared with contacts from low-aerosol (1-9 CFU) and aerosol-negative cases (69%, 25%, and 30%, respectively; P = 0.009). A high-aerosol patient with TB was the only predictor of new M. tuberculosis infection in unadjusted (odds ratio, 5.18; 95% confidence interval, 1.52-17.61) and adjusted analyses (odds ratio, 4.81; 95% confidence interval, 1.20-19.23). Contacts of patients with TB with no aerosols versus low and high aerosols had differential tuberculin skin test and interferon-γ release assay responses.

Conclusions: Cough aerosols of M. tuberculosis are produced by a minority of patients with TB but predict transmission better than sputum smear microscopy or culture. Cough aerosols may help identify the most infectious patients with TB and thus improve the cost-effectiveness of TB control programs.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aerosols*
  • Contact Tracing
  • Cough / microbiology*
  • Family Characteristics
  • Female
  • Humans
  • Interferon-gamma Release Tests / methods
  • Logistic Models
  • Male
  • Mycobacterium tuberculosis / pathogenicity*
  • Predictive Value of Tests
  • Sputum / microbiology*
  • Tuberculin Test
  • Tuberculosis, Pulmonary / complications
  • Tuberculosis, Pulmonary / diagnosis
  • Tuberculosis, Pulmonary / transmission*


  • Aerosols