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. 2013 Jan;31(1):101-110.
doi: 10.1007/s11032-012-9774-z. Epub 2012 Aug 11.

Development of Capsicum EST-SSR Markers for Species Identification and in Silico Mapping Onto the Tomato Genome Sequence

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Free PMC article

Development of Capsicum EST-SSR Markers for Species Identification and in Silico Mapping Onto the Tomato Genome Sequence

Kenta Shirasawa et al. Mol Breed. .
Free PMC article

Abstract

Capsicum spp. are widely cultivated for use as vegetables and spices. The Kihara Institute for Biological Research, Yokohama City University, Japan, has stocks of approximately 800 lines of Capsicum spp. collected from various regions of Central and South America, the regions of origin for Capsicum spp. In this study, 5,751 primer pairs for simple sequence repeat markers, based on 118,060 publicly available sequences of expressed sequence tags of Capsicum annuum, were designed and subjected to a similarity search against the genomic sequence of tomato, a model Solanaceae species. Nucleotide sequences spanning 2,245 C. annuum markers were successfully mapped onto the tomato genome, and 96 of these, which spanned the entire tomato genome, were selected for further analysis. In genotyping analysis, 60 out of the 77 markers that produced specific DNA amplicons showed polymorphism among the Capsicum lines examined. On the basis of the resulting data, the 192 tested lines were grouped into five main clusters. The additional sequencing analysis of the plastid genes, matK and rbcL, divided the resources into three groups. As a result, 19 marker loci exhibited genotypes specific to species and cluster, suggesting that the DNA markers are useful for species identification. Information on the DNA markers will contribute to Capsicum genetics, genomics, and breeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-012-9774-z) contains supplementary material, which is available to authorized users.

Figures

Fig. 1
Fig. 1
Map positions of the CaES markers on the tomato genome. The tomato chromosomes (T01–T12) are indicated in physical length. Vertical bars on the left side of the chromosomes show the heterochromatic regions. Horizontal lines on the chromosomes indicate the positions of the CaES markers; those analyzed in this study are shown with marker names. Descriptions in parentheses following the marker names indicate the marker types: I codominant polymorphic markers, II mixture of codominant and dominant polymorphic markers, III monomorphic markers, IV dominant polymorphic markers, na no amplification or multiple bands
Fig. 2
Fig. 2
A dendrogram of Capsicum species based on genetic distances calculated by the neighbor-joining method. C. annuum, C. baccatum, C. chinense, C. frutescens, C. pubescens, and unclassified Capsicum spp. are shown in red, green, orange, blue, purple, and blackletters, respectively

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