A finger protein structurally similar to TFIIIA that binds exclusively to 5S RNA in Xenopus

Cell. 1990 Apr 20;61(2):293-300. doi: 10.1016/0092-8674(90)90809-s.

Abstract

A 5S RNA binding protein (p43) in Xenopus is a major constituent of oocytes and comprises part of a 42S ribonucleoprotein storage particle. We have cloned and sequenced p43 cDNA from X. laevis and X. borealis as well as the cDNA for X. borealis TFIIIA. Like TFIIIA, p43 has nine zinc fingers, seven of which are exactly the same size as their counterparts in TFIIIA. Amino acid homology between the two proteins is restricted mainly to conserved residues characteristic of zinc fingers. In contrast to TFIIIA, which binds specifically to both 5S RNA and 5S RNA genes, p43 binds exclusively to 5S RNA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cloning, Molecular
  • DNA / genetics
  • DNA / metabolism
  • Female
  • Gene Library
  • Genes
  • Molecular Sequence Data
  • Oocytes / metabolism*
  • Protein Binding
  • RNA, Messenger / genetics
  • RNA, Ribosomal / metabolism*
  • RNA, Ribosomal, 5S / metabolism*
  • RNA-Binding Proteins*
  • Ribonucleoproteins / biosynthesis
  • Ribonucleoproteins / isolation & purification
  • Ribosomal Proteins*
  • Sequence Homology, Nucleic Acid
  • Transcription Factor TFIIIA
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Xenopus Proteins*
  • Xenopus laevis

Substances

  • Carrier Proteins
  • LOC397745 protein, Xenopus
  • RNA, Messenger
  • RNA, Ribosomal
  • RNA, Ribosomal, 5S
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Ribosomal Proteins
  • Transcription Factor TFIIIA
  • Transcription Factors
  • Xenopus Proteins
  • DNA

Associated data

  • GENBANK/M32471
  • GENBANK/M32472
  • GENBANK/M32473