Genetic variants in meiotic program initiation pathway genes are associated with spermatogenic impairment in a Han Chinese population

PLoS One. 2013;8(1):e53443. doi: 10.1371/journal.pone.0053443. Epub 2013 Jan 8.

Abstract

Background: The meiotic program initiation pathway genes (CYP26B1, NANOS1 and STRA8) have been proposed to play key roles in spermatogenesis.

Objective: To elucidate the exact role of the genetic variants of the meiosis initiation genes in spermatogenesis, we genotyped the potential functional genetic variants of CYP26B1, NANOS1 and STRA8 genes, and evaluated their effects on spermatogenesis in our study population.

Design, setting, and participants: In this study, all subjects were volunteers from the affiliated hospitals of Nanjing Medical University between March 2004 and July 2009 (NJMU Infertile Study). Total 719 idiopathic infertile cases were recruited and divided into three groups according to WHO semen parameters: 201 azoospermia patients (no sperm in the ejaculate even after centrifugation), 155 oligozoospermia patients (sperm counts <20×10(6)/ml) and 363 infertility/normozoospermia subjects (sperm counts >20×10(6)/ml). The control group consisted of 383 subjects with normal semen parameters, all of which had fathered at least one child without assisted reproductive technologies.

Measurements: Eight single nucleotide polymorphisms (SNPs) in CYP26B1, NANOS1 and STRA8 genes were determined by TaqMan allelic discrimination assay in 719 idiopathic infertile men and 383 healthy controls.

Results and limitations: The genetic variant rs10269148 of STRA8 gene showed higher risk of spermatogenic impairment in the groups of abnormospermia (including azoospermia subgroup and oligozoospermia subgroup) and azoospermia than the controls with odds ratios and 95% confidence intervals of 2.52 (1.29-4.94) and 2.92 (1.41-6.06), respectively (P = 0.006, 0.002 respective). Notably, larger sample size studies and in vivo or in vitro functional studies are needed to substantiate the biological roles of these variants.

Conclusions: Our results provided epidemiological evidence supporting the involvement of genetic polymorphisms of the meiotic program initiation genes in modifying the risk of azoospermia and oligozoospermia in a Han-Chinese population.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adult
  • Asians / genetics
  • Azoospermia / congenital
  • Case-Control Studies
  • China
  • Cytochrome P-450 Enzyme System / genetics*
  • Genetic Variation / genetics
  • Humans
  • Infertility, Male / genetics*
  • Infertility, Male / pathology
  • Male
  • Meiosis / genetics*
  • Oligospermia / genetics*
  • Oligospermia / pathology
  • Polymorphism, Single Nucleotide
  • Proteins / genetics*
  • RNA-Binding Proteins / genetics*
  • Retinoic Acid 4-Hydroxylase
  • Risk Factors
  • Signal Transduction / genetics*
  • Spermatogenesis / genetics*
  • Young Adult

Substances

  • Adaptor Proteins, Signal Transducing
  • NANOS1 protein, human
  • Proteins
  • RNA-Binding Proteins
  • STRA8 protein, human
  • Cytochrome P-450 Enzyme System
  • CYP26B1 protein, human
  • Retinoic Acid 4-Hydroxylase

Supplementary concepts

  • Arrest of spermatogenesis

Grant support

Funding was provided by grants from National Basic Research Program of China (973 Program) (2009CB941703), the National Natural Science Foundation of China (30930079, 81100461, 810701297 and 30671894), the Jiangsu Natural Science Foundation (BK2011774), Research Fund for the Doctoral Program of Higher Education of China (RFDP) (20113234120001), University natural science research project in Jiangsu Province (11KJB330001) and the Priority Academic Program for the Development of Jiangsu Higher Education Institutions (Public Health and Preventive Medicine). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.