Molecular analysis of an alcohol dehydrogenase-encoding genomic clone (adh2) from rice

Gene. 1990 Mar 15;87(2):185-91. doi: 10.1016/0378-1119(90)90300-g.

Abstract

An alcohol dehydrogenase-encoding gene was isolated from a rice genomic library. The coding region was sequenced, and the gene was identified as adh2. The nucleotide sequence of this gene contains an open reading frame which is interrupted by nine introns. Sequences that are important for eukaryotic gene expression, e.g. the TATA box, polyadenylation signal, intron/exon junction and putative splicing branch sites, are found in the expected locations in the rice adh2. A sequence related to the hexanucleotide core of regulatory elements identified in anaerobically induced plant genes is also present in the 5' region of the rice adh2.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcohol Dehydrogenase / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • Cloning, Molecular
  • Exons
  • Genes, Plant
  • Introns
  • Molecular Sequence Data
  • Oryza / enzymology
  • Oryza / genetics*
  • Promoter Regions, Genetic
  • Protein Conformation
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Alcohol Dehydrogenase

Associated data

  • GENBANK/M36469