Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green

Mycobiology. 2012 Dec;40(4):258-62. doi: 10.5941/MYCO.2012.40.4.258. Epub 2012 Dec 26.


cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H(2)O(2) over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H(2)O(2) improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 µM within 90 min.

Keywords: Dye decolorization; Glyoxal oxidase; Manganese peroxidase; Phanerochaete chrysosporium; Pichia pastoris.