Lamin A/C is expressed in pluripotent mouse embryonic stem cells

Nucleus. Jan-Feb 2013;4(1):53-60. doi: 10.4161/nucl.23384. Epub 2013 Jan 1.


The pluripotent nature of embryonic stem cells (ESC) is associated with a dynamic open chromatin state and an irregular nuclear shape. It has been postulated that the absence of Lamin A/C contributes to these features. However, we show that mouse ESCs express low, yet readily detectable, amounts of Lamin A/C at both the RNA and protein levels. Full-length transcripts of both isoforms were readily detected by q-PCR and deep RNA sequencing. Additionally, protein expression was validated in multiple primary and established ESC lines by immunoblotting using several independent antibodies. Immunofluorescence labeling showed localization of Lamin A/C at the nuclear periphery of all Oct4/Nanog double-positive ESC lines examined, as well as in the inner cell mass of blastocysts. Our results demonstrate ESCs do express low levels of Lamin A/C, thus models linking pluripotency and nuclear dynamics with the absence of Lamin A/C need to be revisited.

Keywords: Lamin A/C; embryonic stem cell; lamina; nucleus.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Blastocyst / metabolism
  • Cell Differentiation
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism*
  • Heterochromatin / metabolism
  • Homeodomain Proteins / metabolism
  • Lamin Type A / genetics
  • Lamin Type A / metabolism*
  • Mice
  • Nanog Homeobox Protein
  • Octamer Transcription Factor-3 / metabolism


  • Heterochromatin
  • Homeodomain Proteins
  • Lamin Type A
  • Nanog Homeobox Protein
  • Nanog protein, mouse
  • Octamer Transcription Factor-3
  • lamin C