Functional analysis of the ATP-binding cassette (ABC) transporter gene family of Tribolium castaneum

BMC Genomics. 2013 Jan 16;14:6. doi: 10.1186/1471-2164-14-6.

Abstract

Background: The ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. Most are integral membrane proteins that transport a broad spectrum of substrates across lipid membranes. In insects, ABC transporters are of special interest because of their role in insecticide resistance.

Results: We have identified 73 ABC transporter genes in the genome of T. castaneum, which group into eight subfamilies (ABCA-H). This coleopteran ABC family is significantly larger than those reported for insects in other taxonomic groups. Phylogenetic analysis revealed that this increase is due to gene expansion within a single clade of subfamily ABCC. We performed an RNA interference (RNAi) screen to study the function of ABC transporters during development. In ten cases, injection of double-stranded RNA (dsRNA) into larvae caused developmental phenotypes, which included growth arrest and localized melanization, eye pigmentation defects, abnormal cuticle formation, egg-laying and egg-hatching defects, and mortality due to abortive molting and desiccation. Some of the ABC transporters we studied in closer detail to examine their role in lipid, ecdysteroid and eye pigment transport.

Conclusions: The results from our study provide new insights into the physiological function of ABC transporters in T. castaneum, and may help to establish new target sites for insect control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / deficiency
  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / metabolism
  • Animals
  • Eye / metabolism
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Genomics*
  • Insect Proteins / deficiency
  • Insect Proteins / genetics*
  • Insect Proteins / metabolism
  • Larva / anatomy & histology
  • Larva / genetics
  • Larva / growth & development
  • Larva / metabolism
  • Lipid Metabolism / genetics
  • Male
  • Phenotype
  • Pigmentation / genetics
  • RNA Interference
  • Tribolium / anatomy & histology
  • Tribolium / genetics*
  • Tribolium / growth & development
  • Tribolium / metabolism

Substances

  • ATP-Binding Cassette Transporters
  • Insect Proteins