Control of plant trichome and root-hair development by a tomato (Solanum lycopersicum) R3 MYB transcription factor

PLoS One. 2013;8(1):e54019. doi: 10.1371/journal.pone.0054019. Epub 2013 Jan 11.

Abstract

In Arabidopsis thaliana the CPC-like MYB transcription factors [CAPRICE (CPC), TRIPTYCHON (TRY), ENHANCER OF TRY AND CPC 1, 2, 3/CPC-LIKE MYB 3 (ETC1, ETC2, ETC3/CPL3), TRICHOMELESS 1, 2/CPC-LIKE MYB 4 (TCL1, TCL2/CPL4)] and the bHLH transcription factors [GLABRA3 (GL3) and ENHANCER OF GLABRA 3 (EGL3)] are central regulators of trichome and root-hair development. We identified TRY and GL3 homologous genes from the tomato genome and named them SlTRY and SlGL3, respectively. Phylogenic analyses revealed a close relationship between the tomato and Arabidopsis genes. Real-time reverse transcription PCR analyses showed that SlTRY and SlGL3 were predominantly expressed in aerial parts of developing tomato. After transformation into Arabidopsis, CPC::SlTRY inhibited trichome formation and enhanced root-hair differentiation by strongly repressing GL2 expression. On the other hand, GL3::SlGL3 transformation did not show any obvious effect on trichome or non-hair cell differentiation. These results suggest that tomato and Arabidopsis partially use similar transcription factors for epidermal cell differentiation, and that a CPC-like R3 MYB may be a key common regulator of plant trichome and root-hair development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis Proteins* / genetics
  • Arabidopsis Proteins* / physiology
  • Basic Helix-Loop-Helix Transcription Factors* / genetics
  • Basic Helix-Loop-Helix Transcription Factors* / metabolism
  • Basic Helix-Loop-Helix Transcription Factors* / physiology
  • DNA-Binding Proteins
  • Gene Expression Regulation, Plant
  • Lycopersicon esculentum / genetics
  • Lycopersicon esculentum / growth & development*
  • Phylogeny
  • Plant Epidermis / growth & development
  • Plant Epidermis / metabolism
  • Plant Roots / genetics
  • Plant Roots / growth & development*
  • Plants, Genetically Modified
  • Transcription Factors / genetics

Substances

  • Arabidopsis Proteins
  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins
  • GL3 protein, Arabidopsis
  • Transcription Factors

Grant support

This work was financially supported by the program "Improvement of Research Environment for Young Researchers" from the Ministry of Education, Culture, Sports, Science and Technology, a grant for Scientific Research on Priority Areas from the University of Miyazaki, a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (No. 23570057) and a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology (No. 23012035). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.