An assay suitable for high throughput screening of anti-influenza drugs

PLoS One. 2013;8(1):e54070. doi: 10.1371/journal.pone.0054070. Epub 2013 Jan 10.

Abstract

We developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts for drug screening. Two potential inhibitors for M2 (V27A) mutant were verified using this method, which inhibit both the mutant and wild-type M2 channels.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antiviral Agents / pharmacology
  • Drug Evaluation, Preclinical
  • Drug Resistance, Viral / drug effects
  • Escherichia coli* / drug effects
  • Escherichia coli* / growth & development
  • Gene Expression Regulation, Bacterial / drug effects
  • High-Throughput Screening Assays
  • Humans
  • Influenza A virus* / drug effects
  • Influenza A virus* / growth & development
  • Influenza, Human / drug therapy*
  • Mutation
  • Recombinant Proteins / antagonists & inhibitors
  • Recombinant Proteins / genetics*
  • Viral Matrix Proteins / antagonists & inhibitors
  • Viral Matrix Proteins / genetics*

Substances

  • Antiviral Agents
  • M2 protein, Influenza A virus
  • Recombinant Proteins
  • Viral Matrix Proteins