The mouse excisional wound splinting model, including applications for stem cell transplantation

Nat Protoc. 2013 Feb;8(2):302-9. doi: 10.1038/nprot.2013.002. Epub 2013 Jan 17.


The mouse excisional wound healing model has been used extensively to study wound healing and cutaneous regeneration. However, as mouse skin is mobile, contraction accounts for a large part of wound closure. In the mouse excisional wound splinting model, a splinting ring tightly adheres to the skin around the wound, preventing local skin contraction. The wound therefore heals through granulation and re-epithelialization, a process similar to that occurring in humans. The model, which takes 2-4 weeks to carry out, can be used to study the effects of stem cells on cutaneous repair or regeneration. In this protocol, we also describe how to implant stem cells onto the wound bed in Matrigel and/or into the surrounding tissue through injection. Serial wound tissue samples at different time points can be harvested to monitor the engraftment and the effects of stem cells in angiogenesis and wound healing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Granulation Tissue / cytology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Models, Animal*
  • Re-Epithelialization / physiology
  • Skin Physiological Phenomena*
  • Splints*
  • Stem Cell Transplantation / methods*
  • Wound Healing / physiology*