Structure of histone mRNA stem-loop, human stem-loop binding protein, and 3'hExo ternary complex

Science. 2013 Jan 18;339(6117):318-21. doi: 10.1126/science.1228705.

Abstract

Metazoan replication-dependent histone messenger RNAs (mRNAs) have a conserved stem-loop (SL) at their 3'-end. The stem-loop binding protein (SLBP) specifically recognizes the SL to regulate histone mRNA metabolism, and the 3'-5' exonuclease 3'hExo trims its 3'-end after processing. We report the crystal structure of a ternary complex of human SLBP RNA binding domain, human 3'hExo, and a 26-nucleotide SL RNA. Only one base of the SL is recognized specifically by SLBP, and the two proteins primarily recognize the shape of the RNA. SLBP and 3'hExo have no direct contact with each other, and induced structural changes in the loop of the SL mediate their cooperative binding. The 3' flanking sequence is positioned in the 3'hExo active site, but the ternary complex limits the extent of trimming.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Catalytic Domain
  • Crystallography, X-Ray
  • Exoribonucleases / chemistry*
  • Histones / chemistry
  • Humans
  • Nuclear Proteins / chemistry*
  • Nucleic Acid Conformation
  • Protein Structure, Tertiary
  • RNA, Messenger / chemistry*
  • Ternary Complex Factors / chemistry*
  • mRNA Cleavage and Polyadenylation Factors / chemistry*

Substances

  • Histones
  • Nuclear Proteins
  • RNA, Messenger
  • SLBP protein, human
  • Ternary Complex Factors
  • mRNA Cleavage and Polyadenylation Factors
  • ERI1 protein, human
  • Exoribonucleases

Associated data

  • PDB/4HXH