Olive oil polyphenols enhance the expression of cholesterol efflux related genes in vivo in humans. A randomized controlled trial

J Nutr Biochem. 2013 Jul;24(7):1334-9. doi: 10.1016/j.jnutbio.2012.10.008. Epub 2013 Jan 17.


Both oleic acid and polyphenols have been shown to increase high-density lipoprotein (HDL) cholesterol and to protect HDL from oxidation, a phenomenon associated with a low cholesterol efflux from cells. Our goal was to determine whether polyphenols from olive oil could exert an in vivo nutrigenomic effect on genes related to cholesterol efflux in humans. In a randomized, controlled, cross-over trial, 13 pre/hypertensive patients were assigned 30 ml of two similar olive oils with high (961 mg/kg) and moderate (289 mg/kg) polyphenol content. We found an increase in ATP binding cassette transporter-A1, scavenger receptor class B type 1, peroxisome proliferator-activated receptor (PPAR)BP, PPARα, PPARγ, PPARδ and CD36 gene expression in white blood cells at postprandial after high polyphenol olive oil when compared with moderate polyphenol olive oil intervention (P<.017), with COX-1 reaching borderline significance (P=.024). Linear regression analyses showed that changes in gene expression were related to a decrease in oxidized low-density lipoproteins and with an increase in oxygen radical absorbance capacity and olive oil polyphenols (P<.05). Our results indicate a significant role of olive oil polyphenols in the up-regulation of genes involved in the cholesterol efflux from cells to HDL in vivo in humans. These results are in agreement with previous ones concerning the fact that benefits associated with polyphenol-rich olive oil consumption on cardiovascular risk could be mediated through an in vivo nutrigenomic effect in humans.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter 1 / genetics
  • Adult
  • Aged
  • Biological Transport
  • CD36 Antigens / genetics
  • Cholesterol / metabolism*
  • Female
  • Gene Expression Regulation / drug effects*
  • Humans
  • Male
  • Middle Aged
  • Olive Oil
  • Peroxisome Proliferator-Activated Receptors / genetics
  • Plant Oils / chemistry*
  • Polyphenols / pharmacology*
  • Scavenger Receptors, Class B / genetics


  • ABCA1 protein, human
  • ATP Binding Cassette Transporter 1
  • CD36 Antigens
  • Olive Oil
  • Peroxisome Proliferator-Activated Receptors
  • Plant Oils
  • Polyphenols
  • SCARB1 protein, human
  • Scavenger Receptors, Class B
  • Cholesterol