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Comparative Study
, 58 (5), 949-55

Modulation of the Fecal Bile Acid Profile by Gut Microbiota in Cirrhosis

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Comparative Study

Modulation of the Fecal Bile Acid Profile by Gut Microbiota in Cirrhosis

Genta Kakiyama et al. J Hepatol.

Abstract

Background & aims: The 7α-dehydroxylation of primary bile acids (BAs), chenodeoxycholic (CDCA) and cholic acid (CA) into the secondary BAs, lithocholic (LCA) and deoxycholic acid (DCA), is a key function of the gut microbiota. We aimed at studying the linkage between fecal BAs and gut microbiota in cirrhosis since this could help understand cirrhosis progression.

Methods: Fecal microbiota were analyzed by culture-independent multitagged-pyrosequencing, fecal BAs using HPLC and serum BAs using LC-MS in controls, early (Child A) and advanced cirrhotics (Child B/C). A subgroup of early cirrhotics underwent BA and microbiota analysis before/after eight weeks of rifaximin.

Results: Cross-sectional: 47 cirrhotics (24 advanced) and 14 controls were included. In feces, advanced cirrhotics had the lowest total, secondary, secondary/primary BA ratios, and the highest primary BAs compared to early cirrhotics and controls. Secondary fecal BAs were detectable in all controls but in a significantly lower proportion of cirrhotics (p<0.002). Serum primary BAs were higher in advanced cirrhotics compared to the rest. Cirrhotics, compared to controls, had a higher Enterobacteriaceae (potentially pathogenic) but lower Lachonospiraceae, Ruminococcaceae and Blautia (7α-dehydroxylating bacteria) abundance. CDCA was positively correlated with Enterobacteriaceae (r=0.57, p<0.008) while Ruminococcaceae were positively correlated with DCA (r=0.4, p<0.05). A positive correlation between Ruminococcaceae and DCA/CA (r=0.82, p<0.012) and Blautia with LCA/CDCA (r=0.61, p<0.03) was also seen. Prospective study: post-rifaximin, six early cirrhotics had reduction in Veillonellaceae and in secondary/primary BA ratios.

Conclusions: Cirrhosis, especially advanced disease, is associated with a decreased conversion of primary to secondary fecal BAs, which is linked to abundance of key gut microbiome taxa.

Conflict of interest statement

Conflicts of interest: JSB is a consultant for Salix Pharmaceuticals

Figures

Fig 1
Fig 1. Change in fecal secondary/primary bile acid concentration ratios after rifaximin therapy in early cirrhotic patients
Figure 1a: LCA+DCA/CDCA+CA ratio, Figure 1b: LCA/CDCA ratio and Figure 1c: DCA/CA ratio change; there was a significant decrease in the fecal secondary to primary bile acid ratios after rifaximin therapy in early cirrhotic patients

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