Oct-4 is required for an antiapoptotic behavior of chemoresistant colorectal cancer cells enriched for cancer stem cells: effects associated with STAT3/Survivin

Cancer Lett. 2013 Jun 1;333(1):56-65. doi: 10.1016/j.canlet.2013.01.009. Epub 2013 Jan 20.


Cancer stem cells (CSCs) have been implicated in multidrug resistance, a phenomenon responsible for the failure of cancer chemotherapy. However, the underlying mechanisms are still unclear. In our study, we established two oxaliplatin-resistant colorectal cancer cell lines displaying some CSCs characteristics. Oct4 overexpression was observed in these two lines. We performed Oct4 knock down by lentiviral vector-mediated specific shRNA. Knockdown increased apoptosis, decreased CSCs marker expression and weakened tumorigenicity in drug-resistant cell lines. In conclusion, we show that these events can be at least in part attributed to the STAT3/Survivin pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AC133 Antigen
  • Animals
  • Antigens, CD / analysis
  • Apoptosis*
  • Cell Line, Tumor
  • Colorectal Neoplasms / drug therapy*
  • Colorectal Neoplasms / pathology
  • Drug Resistance, Neoplasm
  • Glycoproteins / analysis
  • Humans
  • Hyaluronan Receptors / analysis
  • Inhibitor of Apoptosis Proteins / physiology*
  • Mice
  • Mice, SCID
  • Neoplastic Stem Cells / chemistry
  • Neoplastic Stem Cells / physiology*
  • Octamer Transcription Factor-3 / physiology*
  • Organoplatinum Compounds / pharmacology
  • Oxaliplatin
  • Peptides / analysis
  • STAT3 Transcription Factor / physiology*
  • Survivin


  • AC133 Antigen
  • Antigens, CD
  • BIRC5 protein, human
  • Glycoproteins
  • Hyaluronan Receptors
  • Inhibitor of Apoptosis Proteins
  • Octamer Transcription Factor-3
  • Organoplatinum Compounds
  • POU5F1 protein, human
  • Peptides
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • Survivin
  • Oxaliplatin