Rapid characterization of hydrogen exchange in proteins

Angew Chem Int Ed Engl. 2013 Feb 25;52(9):2440-3. doi: 10.1002/anie.201206828. Epub 2013 Jan 22.

Abstract

Kinetics and thermodynamics of amide hydrogen exchange in proteins can be investigated with two-dimensional (13)CO-(15)N NMR correlation experiments. The spectra are acquired with high resolution and sensitivity. A single type of experiment on one sample serves to characterize hydrogen-deuterium fractionation factors and hydrogen-exchange rates that span three orders of magnitude.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amides / chemistry
  • Amides / metabolism
  • Hydrogen / chemistry*
  • Hydrogen / metabolism
  • Kinetics
  • Models, Molecular
  • Protein Folding
  • Proteins / chemistry*
  • Proteins / metabolism
  • Thermodynamics
  • Ubiquitin / chemistry
  • Ubiquitin / metabolism

Substances

  • Amides
  • Proteins
  • Ubiquitin
  • Hydrogen